Profile: Wako Chemicals GmbH manufactures diagnostic reagents for clinical chemistry. Lipids play an important role in cell metabolism. Since the hydrophobic character of lipids prevents their direct transport in plasma, they are transported in the form of particles known as lipoproteins. Each lipoprotein contains protein components called apolipoproteins. Apolipoproteins helps in maintaining the structural integrity and stability of lipoprotein particles. In addition, apolipoproteins perform various functions in the metabolic conversion of lipoproteins. Serum bilirubin measurement is widely used as a screening test for liver functions. The common methods most widely used for determination of serum bilirubin are the diazo coupling method and the bilirubin oxidase enzymatic method .