Profile: Takara Bio Inc. focuses on genetic engineering. Our cycling probe technology is a high-sensitive detection method utilizing a combination of chimera probe, composed of RNA and DNA, and RNase H. The specific sequence of target gene to be amplified can be detected efficiently during or after amplification by this method. One end of the probe is labeled with a fluorescent substance and the other end is labeled with a quencher, which quenches the fluorescence emitted from the fluorescent substance. As long as this probe remains intact, no strong fluoescence can be emitted because of the quenching function. When this probe forms a hybrid with the complementary sequence of amplified product, RNase H specifically cuts the RNA region of this probe, resulting in emission of strong fluorescence. By measuring the intensity of emitted fluorescence, the amount of amplified product can be monitored. Our protein molecular weight markers are designed for use in SDS-polyacrylamide gel electrophoresis. Our product calpastatin is an endogenous protease inhibitor that acts specifically on calpain. It consists of four repetitive sequences of 120 to 140 amino acid residues & an N-terminal non-homologous sequence (L). This product is obtained from highly purified domain I of human calpastatin comprising of amino acids which is produced by genetic recombination technique.