A database was developed to store, organize & retrieve the data associated with electrophoresis and chromatography separations. It allows laboratories to store extensive data on separation techniques (analytical & preparative). The data for gel electrophoresis includes gel composition, staining methods, electric fields, analysis & samples loaded. The database stores data on chromatography conditions, the samples used and the fractions collected. The data structure of this database was designed to maintain the link between samples (including fractions) from chromatography separations and their analysis by gel electrophoresis. The database will allow laboratories to organize and maintain a large amount of separation & sample data in a uniform data environment. It will facilitate the retrieval of the separation history of important samples and the separation conditions used.