Profile: Bioland Scientific LLC is a laboratory supply and biotechnology company. Our products include transfection reagent, plasmid DNA purification kit, buffers & ezFilters, virus purification kit, centrifuge tubes, genomic DNA purification kit, gloves, DNA fragment purification kit and X-ray films. BioT is a lipid-based reagent designed and formulated with proprietary technology for transfecting DNA/siRNA into a variety of eukaryotic cell lines including HEK293, CHO, COS, NIH3T3, PC12, RKO, C2C12 myoblasts, mouse embryonic stem cells, S2 drosophial cells as well as primary cultures of human carcinoma, rat adrenal chromaffin cells, cortical neurons & hepatocytes. It also offers extremely high transfection efficiencies and minimal cytotoxicity for suspension cultures including HEK 293, mouse lymphocytes & insect cells. The reagent works very well in cultures with serum concentration ranging from 0 to 20% FBS. Our patented plasmid purification kit uses special buffer system and the resulting purified DNA is free of guanidine salt, cesium chloride, ethidium bromide & ion exchange resin residues which enable the highest performance of downstream applications including gene therapy & genetic vaccination. Our plasmid mini kits provide an easy and reliable method to purify plasmid DNA from 1 ml to 5 ml bacterial cultures in less than 20 minute. The DNA is ready for downstream applications such as restriction digestions, PCR and automated sequencing.