Desizing is the process of removing the size material from the warp yarns in woven fabrics. Enzymatic desizing is the classical desizing process of degrading starch size on cotton fabrics using enzymes. Enzymes are complex organic, soluble bio-catalysts, formed by living organisms, that catalyze chemical reaction in biological processes. Enzymes are quite specific in their action on a particular substance. A small quantity of enzyme is able to decompose a large quantity of the substance it acts upon. Enzymes are usually named by the kind of substance degraded in the reaction it catalyzes. Amylases are the enzymes that hydrolyses and reduce the molecular weight of amylose and amylopectin molecules in starch, rendering it water soluble enough to be washed off the fabric. Effective enzymatic desizing require strict control of pH, temperature, water hardness, electrolyte addition and choice of surfactant.